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KMID : 0377619750290020197
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Korean Jungang Medical Journal
1975 Volume.29 No. 2 p.197 ~ p.208
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Effect of Reduced Glutathion on the Pulmonary Surfactant Activities of the Rabbit Following Carbon Tetrachloride Intoxication
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蔡洙喜/Chae, Soo Hi
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Abstract
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The present study was undertaken to observe a possible damaging effect of carbon tetrachloride (CCl4) on the pulmonary surfactant activities, and to elucidate the effectiveness of reduced glutathione (GSH) on the pulmonary surfactant activities of the CC], intoxicated animal.
The rabbit was used throughout the entire experiment, and the mixture of the surfactant activities of the lung washings were observed one day after the respective CCl4 administration as a preliminary experiment to determine the appropriate dose of CC14 in the main experiment.
All the experimental data obtained from the preliminary study showed decreased surfactant activities from the control, but when CCl4 mixture of 0. 3ml per Kg BW was administered twice weekly for one week, the decrease was not as prominent as when higher doses of CC14 were used, in which cases the decrease -was similar.
From the above results, CC14 mixture of 0.3m1 per Kg BW was used twice weekly for two weeks in the main experiment, and the changes of the maximum and minimum surface tension, and stability index were studied on 1, 3 and 5 days after the CC], administration.
The changes of the body weight and left lung weight were also measured, and the left lung weight/body weight ratio was thus calculated. GSH in the dose of 20mg per Kg BW was intraperitoneally administered either singularly or in combination with CC14i and the study was performed as in the case of CCLadministration.
The results thus obtained are summarized as follows:
1) The maximum and minimum surface tension and stability index of the normal rabbit lung washings was 56.1´2.5 dyne/ cm, 5.5±1.2 dyne/cm and 1.70, respectively.
The protein content of the lung washings was 100.3 ´ 8.5mg/mI and the body weight, left lung ´eight and left lung weight/body weight ratio was 2.23±0.17 kg, 3.0±0.27gm and 1.35±0.15, respectively in the normal rabbit.
2) When 0.3m1 of the mixture of CC14 and olive oil per Kg BW was admini￢stered twice weekly for one week, the surfactant activities of the lung washings did not change noticeably from the control values, but the surfactant activities decreased when 0.3m1 of CCI4 was administered twice weekly for two weeks: and the tendency was similar when the dosage of CCI4 was increased to 0.5m] for one or two weeks.
3) While administration of 0.3m1 of CCI4 alone twice weekly for two weeks, produced a decreased surfactant activities, GSH caused no change from the control. When GSH and CC14 were combined, the surfactant activities were lower than the GSH group, but higher than the CC", group.
4) The decrease of the body weight by CCI4 administration was prominent but left lung weight did not change noticeably. Thus, the left lung weight ratio was elevated from the control throughout the experiment. The protein contents, of the lung washings in the CC14 intoxicated group showed decreased values from the control. When GSH alone was used, the body weight, left lung weight, left lung weight/body weight ratio and protein content of the lung washings did not change from the control, but. the combined administration of GSH and CC14: produced higher values than the CCI4 group.
5) From the above, it may be concluded that CCI4 produces lowered surfactant activities. Also, GSH seems to be effective to some extent to minimize the damaging effect of CCI4 on the pulmonary surfactant activities
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KEYWORD
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